3413 pBF339 Bcl-XL
(Plasmid #8773)

• Depositing Lab: Stanley Korsmeyer
• Publication: Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Backbone

• Vector backbone: pBF339
• Backbone size w/o insert (bp): 6680
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl-XL
• Species: M. musculus (mouse)
• Insert Size (bp): 700
• Entrez Gene: Bcl2l1 (a.k.a. Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-x, bcl2-L-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: HA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA encoding murine BCL-XL into EcoRI site directly downstream of yeast ADH1 promoter, also 2um origin of replication