pUC57 DYSF, alt exon 1a, 5a, 17
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87896||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepUC57 Kan
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameDYSF, alt exon 1a, 5a, 17
SpeciesH. sapiens (human)
Mutationalternative isoforms, see comments
Entrez GeneDYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC57 DYSF, alt exon 1a, 5a, 17 was a gift from Jain Foundation (Addgene plasmid # 87896 ; http://n2t.net/addgene:87896 ; RRID:Addgene_87896)