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pC-HBH
(Plasmid #88896)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 88896 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Promoter CAG
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Low Kan (25ug/mL)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    Depositor recommends DH10B, XL1 blue or NEB's DH10-beta for growth. DH5alpha should not be used as it does not yield good transformation results
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC-HBH was a gift from Michele Calos (Addgene plasmid # 88896 ; http://n2t.net/addgene:88896 ; RRID:Addgene_88896)
  • For your References section:

    Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells. Zhao C, Farruggio AP, Bjornson CR, Chavez CL, Geisinger JM, Neal TL, Karow M, Calos MP. PLoS One. 2014 Apr 29;9(4):e96279. doi: 10.1371/journal.pone.0096279. eCollection 2014. 10.1371/journal.pone.0096279 PubMed 24781921