Gal4 PGC1 alpha 1-550
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8894||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCMX Gal4
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePGC-1 alpha
Alt namePPAR gamma coactivator 1
SpeciesM. musculus (mouse)
Insert Size (bp)1650
Mutationamino acids 1-550 of PGC-1 alpha
Entrez GenePpargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
/ Fusion Protein
- Gal4 DBD (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer GAGTAGTAACAAAGGTCAA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
PGC1(1-550) was cloned downstream of the Gal4 DNA binding domain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Gal4 PGC1 alpha 1-550 was a gift from Bruce Spiegelman (Addgene plasmid # 8894 ; http://n2t.net/addgene:8894 ; RRID:Addgene_8894)
For your References section:Activation of PPARgamma coactivator-1 through transcription factor docking. Puigserver P, Adelmant G, Wu Z, Fan M, Xu J, O'Malley B, Spiegelman BM. Science 1999 Nov 12;286(5443):1368-71. 10.1126/science.286.5443.1368 PubMed 10558993