Purposemammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids linked to VP16 activation domain
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89081||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Mutationaa 1-660 (contains exons A,B,C and part of D)
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter SV40
/ Fusion Protein
- VP16 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
This vector contains a short VP16 activation domain (AA 411-456.)
Please also see https://www.ncbi.nlm.nih.gov/pubmed/10816582.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:VP-hAR1-660 was a gift from Elizabeth Wilson (Addgene plasmid # 89081 ; http://n2t.net/addgene:89081 ; RRID:Addgene_89081)
For your References section:Intermolecular NH2-/carboxyl-terminal interactions in androgen receptor dimerization revealed by mutations that cause androgen insensitivity. Langley E, Kemppainen JA, Wilson EM. J Biol Chem. 1998 Jan 2;273(1):92-101. PubMed 9417052