This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p5M-PR-B-1-688
(Plasmid #89115)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89115 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human progesterone receptor-B N-term+DNA binding domain
  • Species
    H. sapiens (human)
  • Mutation
    5 restriction sites deleted in polylinker, contains aa 1-688
  • Entrez Gene
    PGR (a.k.a. NR3C3, PR)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The 5 restriction sites deleted from the pCMV5 polylinker are HindIII, PstI, SalI, BamHI, and XbaI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5M-PR-B-1-688 was a gift from Elizabeth Wilson (Addgene plasmid # 89115 ; http://n2t.net/addgene:89115 ; RRID:Addgene_89115)
  • For your References section:

    Gain in transcriptional activity by primate-specific coevolution of melanoma antigen-A11 and its interaction site in androgen receptor. Liu Q, Su S, Blackwelder AJ, Minges JT, Wilson EM. J Biol Chem. 2011 Aug 26;286(34):29951-63. doi: 10.1074/jbc.M111.244715. Epub 2011 Jul 5. 10.1074/jbc.M111.244715 PubMed 21730049