pPS1739
(Plasmid #8922)

• Depositing Lab: Pamela Silver
• Publication: Ferrigno et al EMBO J. 1998 Oct 1. 17(19):5606-14.

Backbone

• Vector backbone: pRS-GFP
• Backbone size w/o insert (bp): 5800
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HOG1
• Alt name: HOG1-GFP
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: HOG1 (a.k.a. YLR113W, SSK3)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pRS-GFP vector is GFP ORF and NUF2 3' UTR from pPS934 cloned into the XhoI-KpnI sites of pRS416. HOG1 (from -290 to the termination codon) was amplified by PCR using primers that replaced the termination codon with a XhoI site. CEN plasmid.