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pPS2966
(Plasmid #8925)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8925 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NDR1
  • Alt name
    pGST-NDR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    STK38 (a.k.a. NDR, NDR1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer pGEX5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NDR1 ORF PCR amplified from Hela cDNA. ~1.5 kb insert digested with BamHI/ScaI and inserted into pGEX-4T1 digested with BamHI/SmaI. Completely sequenced and includes the entire NDR ORF as reported in NCBI database. Note: GST-NDR1 is extremely insoluble when expressed in E. coli, possibility that co-expression of Mob2 would help solubilize NDR1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2966 was a gift from Pamela Silver (Addgene plasmid # 8925 ; http://n2t.net/addgene:8925 ; RRID:Addgene_8925)
  • For your References section:

    Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. Devroe E, Erdjument-Bromage H, Tempst P, Silver PA. J Biol Chem. 2004 Jun 4. 279(23):24444-51. 10.1074/jbc.M401999200 PubMed 15067004