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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepECMV-FLAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNDR2
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Alt namepFLAG-NDR2
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SpeciesH. sapiens (human)
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Entrez GeneSTK38L (a.k.a. NDR2)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NDR2 ORF PCR amplified from Hela cDNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS2991 was a gift from Pamela Silver (Addgene plasmid # 8931 ; http://n2t.net/addgene:8931 ; RRID:Addgene_8931) -
For your References section:
Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. Devroe E, Erdjument-Bromage H, Tempst P, Silver PA. J Biol Chem. 2004 Jun 4. 279(23):24444-51. 10.1074/jbc.M401999200 PubMed 15067004