|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89332||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5642
- Total vector size (bp) 7155
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Insert Size (bp)1212
GenBank IDNM_000314 NM_000314
Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
- Promoter CMV
/ Fusion Protein
- C-terminal part of Venus (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site AflII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer bGH (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Original clone (alpha Synuclein-VC) includes already the C-terminal part of venus, and was a gift from Pamela J.McLean.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PTEN-VC was a gift from Tiago Outeiro (Addgene plasmid # 89332 ; http://n2t.net/addgene:89332 ; RRID:Addgene_89332)