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deltaPH Dynamin-2-mNeon siRNA-resistant variant
(Plasmid #89338)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89338 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pmNeonGreen-N1
  • Backbone manufacturer
    Allele Biotechnology & Pharmaceuticals, Inc
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dynamin-2 isoform 3 with silent mutations and deleted PH domain
  • Alt name
    Resistant to siRNA #1 targeting GAGATCAGGTGGACACTCT
  • Alt name
    Resistant to siRNA #2 targeting GAGCGAATCGTCACCACTT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2289
  • Mutation
    A1044T, T1047C, G1050A, G1053C, C1056T, T1059C, G1371A, A1374C, C1377A, C1380A, C1383A, T1386G + deletion of bases 1558 to 1869
  • GenBank ID
    NM_004945
  • Promoter CMV
  • Tag / Fusion Protein
    • pmNeonGreen-N1 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGATC CTCGAG ATG GGC AAC CGC GGG ATG GAA GAG
  • 3′ sequencing primer CAGATC GAATTC G GTC GAG CAG GGA TGG CTC GGC
  • (Common Sequencing Primers)
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    deltaPH Dynamin-2-mNeon siRNA-resistant variant was a gift from Gia Voeltz (Addgene plasmid # 89338)

  • For your References section:

    Multiple dynamin family members collaborate to drive mitochondrial division. Lee JE, Westrate LM, Wu H, Page C, Voeltz GK. Nature. 2016 Dec 1;540(7631):139-143. doi: 10.1038/nature20555. Epub 2016 Oct 31. 10.1038/nature20555 PubMed 27798601
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