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pLL3.7m-psMEK1
(Plasmid #89361)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89361 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLL3.7m
  • Backbone manufacturer
    Luk Parijs
  • Backbone size w/o insert (bp) 6500
  • Modifications to backbone
    5' and 3' UTRs of HIV transcript reduced while retaining essential packaging elements
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    psMEK1
  • Alt name
    photoswitchable MEK1
  • Alt name
    photoswitchable MAPKK1
  • Alt name
    photoswitchable MAP2K1
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MKK1, PRKMK1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • pdDronpa
    • Human influenza hemagglutinin (HA) tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGATAACAATTTCACACAG
  • 3′ sequencing primer CGGCCTTTTTACGGTTCCTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7m-psMEK1 was a gift from Michael Lin (Addgene plasmid # 89361 ; http://n2t.net/addgene:89361 ; RRID:Addgene_89361)
  • For your References section:

    Optical control of cell signaling by single-chain photoswitchable kinases. Zhou XX, Fan LZ, Li P, Shen K, Lin MZ. Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605. 10.1126/science.aah3605 PubMed 28232577