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pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE
(Plasmid #89607)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89607 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CCLc
  • Backbone manufacturer
    Luigi Naldini, PMID 9765382
  • Backbone size w/o insert (bp) 8499
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LIN28A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    633
  • GenBank ID
    NM_024674.4
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
  • Promoter MNDU3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LIN28 was sub-cloned from the plasmid pSin-EF2-LIN28-Pur from James Thomson (Addgene plasmid # 16580).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE was a gift from Fernando Fierro (Addgene plasmid # 89607 ; http://n2t.net/addgene:89607 ; RRID:Addgene_89607)
  • For your References section:

    Fibroblast Growth Factor 2 Regulates High Mobility Group A2 Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells. Kalomoiris S, Cicchetto AC, Lakatos K, Nolta JA, Fierro FA. J Cell Biochem. 2016 Sep;117(9):2128-37. doi: 10.1002/jcb.25519. Epub 2016 Mar 8. 10.1002/jcb.25519 PubMed 26888666