pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE
(Plasmid #89607)

• Purpose: Expression of LIN28A in human cells
• Depositing Lab: Fernando Fierro
• Publication: Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37. doi: 10.1002/jcb.25519. Epub 2016 Mar 8.

Backbone

• Vector backbone: CCLc
• Backbone manufacturer: Luigi Naldini, PMID 9765382
• Backbone size w/o insert (bp): 8499
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LIN28A
• Species: H. sapiens (human)
• Insert Size (bp): 633
• GenBank ID: NM_024674.4
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
• Promoter: MNDU3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

LIN28 was sub-cloned from the plasmid pSin-EF2-LIN28-Pur from James Thomson (Addgene plasmid # 16580).