pCDF-casBCDE(+6)
(Plasmid #89728)

• Purpose: Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.
• Depositing Labs: Konstantin Kuznedelov, Konstantin Severinov
• Publication: Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10849-10861. Epub 2016 Oct 13.

Backbone

• Vector backbone: pCDF-casBCDE
• Backbone manufacturer: PMID:26013814
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRISPR array
• Alt name: CRISPR mini-cassette
• Mutation: Derivative CRISPR array containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • pCDF-casBCDE(+6).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry