PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89744||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDerived by our lab using pExFP-C1 and pGL3
- Backbone size w/o insert (bp) 5673
- Total vector size (bp) 6119
Vector typeMammalian Expression, Luciferase
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt nameAsLov2Jalpha (404-546)-JIP11
SpeciesSynthetic; Avena Sativa and
Insert Size (bp)478
- Promoter CMV
/ Fusion Protein
- Firefly luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer luc2131+
- 3′ sequencing primer fastbac reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byWe designed and assembled the insert from de novo synthesised sequences
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017 in press.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLuc-OptoJNKi was a gift from Michael Courtney (Addgene plasmid # 89744 ; http://n2t.net/addgene:89744 ; RRID:Addgene_89744)
For your References section:A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Melero-Fernandez de Mera RM, Li LL, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ. Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017. 10.1038/ncomms15017 PubMed 28497795