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pYpet-Optop38i
(Plasmid #89749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89749 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYpet-C1
  • Backbone manufacturer
    Derived by our lab using pExFP-C1 and JNKAREV
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 5180
  • Vector type
    Mammalian Expression ; Fluorescent protein
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Optop38i3
  • Alt name
    AsLov2Jalpha (404-546)-3-13F
  • Species
    Synthetic; Avena Sativa and
  • Insert Size (bp)
    481
  • GenBank ID
    KY595946
  • Promoter CMV
  • Tag / Fusion Protein
    • Ypet (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer YFP_Venus-fwd
  • 3′ sequencing primer fastbac reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We designed and assembled the insert from de novo synthesised sequences
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017 in press.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYpet-Optop38i was a gift from Michael Courtney (Addgene plasmid # 89749 ; http://n2t.net/addgene:89749 ; RRID:Addgene_89749)
  • For your References section:

    A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Melero-Fernandez de Mera RM, Li LL, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ. Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017. 10.1038/ncomms15017 PubMed 28497795