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Addgene

pLenti6-CENP-A-mCherry
(Plasmid #89767)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89767 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti6/V5-DEST
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8300
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CENP-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    CENPA (a.k.a. CENP-A, CenH3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xHA (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6-CENP-A-mCherry was a gift from Torsten Wittmann (Addgene plasmid # 89767 ; http://n2t.net/addgene:89767 ; RRID:Addgene_89767)
  • For your References section:

    GSK3-mediated CLASP2 phosphorylation modulates kinetochore dynamics. Pemble H, Kumar P, van Haren J, Wittmann T. J Cell Sci. 2017 Apr 15;130(8):1404-1412. doi: 10.1242/jcs.194662. Epub 2017 Feb 23. 10.1242/jcs.194662 PubMed 28232523