Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

isoform PKC Alpha (JCE599)
(Plasmid #89779)


Item Catalog # Description Quantity Price (USD)
Plasmid 89779 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    pProm-PKC1-Adh1t is YCplac33 with yeast PKC1 promoter and Adh1t terminator
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    PKC Alpha
  • Alt name
    protein kinase C, alpha
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prkca (a.k.a. Pkca)
  • Promoter yeast PKC1 promoter (-600 to -1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-Rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    isoform PKC Alpha (JCE599) was a gift from Juan Carlos Igual (Addgene plasmid # 89779 ; ; RRID:Addgene_89779)
  • For your References section:

    Protein kinase C controls activation of the DNA integrity checkpoint. Soriano-Carot M, Quilis I, Bano MC, Igual JC. Nucleic Acids Res. 2014 Jun;42(11):7084-95. doi: 10.1093/nar/gku373. Epub 2014 May 3. 10.1093/nar/gku373 PubMed 24792164