Purpose(Empty Backbone) BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSG
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90273||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3147
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Unknown
- 5′ sequencing primer CATTAATGCAGCTGGCAC
- 3′ sequencing primer GGTTATTGTCTCATGAGCGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene's sequencing results found that the plasmid backbone differs from other BB2_L_12 constructs in this paper. The depositor has confirmed that this difference should not affect functionality.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BB2_L_12_syn_A_G was a gift from Michael Sauer (Addgene plasmid # 90273 ; http://n2t.net/addgene:90273 ; RRID:Addgene_90273)
For your References section:An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. 10.1016/j.biortech.2017.05.004 PubMed 28533066