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An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger.
Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89914BB1_L_12_syn_BsaILinker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)
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89915BB1_L_23_syn_BsaILinker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI)
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89916BB1_L_34_syn_BsaILinker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)
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89917BB2_L_AB_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)
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89918BB2_L_BC_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)
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89919BB2_L_CD_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)
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89920BB2_L_DE_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)
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89921BB2_L_EF_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)
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89922BB2_L_FG_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)
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89923BB2_L_GH_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)
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89924BB2_L_HI_syn_BbsILinker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI)
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90268BB2_L_12_syn_A_BBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSB
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90269BB2_L_12_syn_A_CBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSC
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90270BB2_L_12_syn_A_DBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSC
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90271BB2_L_12_syn_A_EBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSE
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90272BB2_L_12_syn_A_FBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSF
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90273BB2_L_12_syn_A_GBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSG
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90274BB2_L_12_syn_A_HBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSH
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90275BB2_L_12_syn_A_IBB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSI
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90276pCAS_gpyrG1(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9
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90277pCAS_gpyrG2(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9
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90278pMST665_BB3_L 23_gRNAempty_cas9_BbsIBB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9
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90279pMST634-BB3_L_AB_hyg_KanOri_pyrG5BB3 recipient for homologous integration in pyrG locus with linker AB
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90280pMST635- BB3_L_AC_hyg_KanOri_pyrG5BB3 recipient for homologous integration in pyrG locus with linker AC
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90281pMST636_BB3_L_AD_hyg_KanOri_pyrG5BB3 recipient for homologous integration in pyrG locus with linker AD
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90282pMST637_BB3_L_AE_hyg_KanOri_pyrG5BB3 recipient for homologous integration in pyrG locus with linker AE
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90283pMST638_BB3_L_AF_hyg_KanOri_pyrG5BB3 recipient for homologous integration in pyrG locus with linker AF
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90284pMST639_BB3_L_AG_hyg_KanOri_pyrG5BB3 recipient for homologous integration in pyrG locus with linker AG
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90285pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8BB2 with split pyrG marker for homologous integration with linker AB (negative control)
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90286pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)
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90287pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)
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90288pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)
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90289BB1_P_12_asn_mbfABB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2
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90290BB1_P_12_asn_coxABB1 promoter coxA gene of Aspergillus niger between FS1 and FS2
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90291BB1_T_34_ani_trpCBB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4
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90292pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance
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90293BB1_O_34_AMA1_2.8BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. niger between FS 34
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