An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger.
Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. PubMed Article
Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89914 | BB1_L_12_syn_BsaI | Linker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI) |
| 89915 | BB1_L_23_syn_BsaI | Linker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI) |
| 89916 | BB1_L_34_syn_BsaI | Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI) |
| 89917 | BB2_L_AB_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI) |
| 89918 | BB2_L_BC_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI) |
| 89919 | BB2_L_CD_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI) |
| 89920 | BB2_L_DE_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI) |
| 89921 | BB2_L_EF_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI) |
| 89922 | BB2_L_FG_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI) |
| 89923 | BB2_L_GH_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI) |
| 89924 | BB2_L_HI_syn_BbsI | Linker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI) |
| 90268 | BB2_L_12_syn_A_B | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSB |
| 90269 | BB2_L_12_syn_A_C | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSC |
| 90270 | BB2_L_12_syn_A_D | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSC |
| 90271 | BB2_L_12_syn_A_E | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSE |
| 90272 | BB2_L_12_syn_A_F | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSF |
| 90273 | BB2_L_12_syn_A_G | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSG |
| 90274 | BB2_L_12_syn_A_H | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSH |
| 90275 | BB2_L_12_syn_A_I | BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSI |
| 90276 | pCAS_gpyrG1 | (also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9 |
| 90277 | pCAS_gpyrG2 | (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9 |
| 90278 | pMST665_BB3_L 23_gRNAempty_cas9_BbsI | BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9 |
| 90279 | pMST634-BB3_L_AB_hyg_KanOri_pyrG5 | BB3 recipient for homologous integration in pyrG locus with linker AB |
| 90280 | pMST635- BB3_L_AC_hyg_KanOri_pyrG5 | BB3 recipient for homologous integration in pyrG locus with linker AC |
| 90281 | pMST636_BB3_L_AD_hyg_KanOri_pyrG5 | BB3 recipient for homologous integration in pyrG locus with linker AD |
| 90282 | pMST637_BB3_L_AE_hyg_KanOri_pyrG5 | BB3 recipient for homologous integration in pyrG locus with linker AE |
| 90283 | pMST638_BB3_L_AF_hyg_KanOri_pyrG5 | BB3 recipient for homologous integration in pyrG locus with linker AF |
| 90284 | pMST639_BB3_L_AG_hyg_KanOri_pyrG5 | BB3 recipient for homologous integration in pyrG locus with linker AG |
| 90285 | pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8 | BB2 with split pyrG marker for homologous integration with linker AB (negative control) |
| 90286 | pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8 | BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette) |
| 90287 | pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8 | BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes) |
| 90288 | pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8 | BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes) |
| 90289 | BB1_P_12_asn_mbfA | BB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2 |
| 90290 | BB1_P_12_asn_coxA | BB1 promoter coxA gene of Aspergillus niger between FS1 and FS2 |
| 90291 | BB1_T_34_ani_trpC | BB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4 |
| 90292 | pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8 | BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance |
| 90293 | BB1_O_34_AMA1_2.8 | BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. niger between FS 34 |