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pMST665_BB3_L 23_gRNAempty_cas9_BbsI
(Plasmid #90278)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90278 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    BB3_AMA_2.8_pUC-ORI_L_AC_hph
  • Backbone size w/o insert (bp) 5997
  • Total vector size (bp) 14521
  • Vector type
    Bacterial Expression ; A. niger

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9
  • gRNA/shRNA sequence
    -

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMST665_BB3_L 23_gRNAempty_cas9_BbsI was a gift from Michael Sauer (Addgene plasmid # 90278 ; http://n2t.net/addgene:90278 ; RRID:Addgene_90278)
  • For your References section:

    An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. 10.1016/j.biortech.2017.05.004 PubMed 28533066