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pKD233.7-3
(Plasmid #90955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90955 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ColE1
  • Total vector size (bp) 5437
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Tetrathionate response regulator
  • Alt name
    TtrR
  • Species
    Shewanella baltica OS195
  • Insert Size (bp)
    621
  • GenBank ID
    ABX51018
  • Promoter PltetO-1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Superfolder GFP
  • Alt name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter PttrB185-269

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter Bba_J23114

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results found A82V and G196S mutations in mCherry and a K140M mutation in sfGFP. The depositing lab confirmed that mCherry is functional as a constitutive marker, and that sfGFP fluorescence increases upon sensor activation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD233.7-3 was a gift from Jeffrey Tabor (Addgene plasmid # 90955 ; http://n2t.net/addgene:90955 ; RRID:Addgene_90955)
  • For your References section:

    Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Daeffler KN, Galley JD, Sheth RU, Ortiz-Velez LC, Bibb CO, Shroyer NF, Britton RA, Tabor JJ. Mol Syst Biol. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416. PubMed 28373240