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Addgene

pKD184
(Plasmid #90953)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90953 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ColE1
  • Total vector size (bp) 5684
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Thiosulfate response regulator
  • Alt name
    ThsR
  • Species
    Shewanella halifaxensis HAW-EB4
  • Insert Size (bp)
    612
  • GenBank ID
    ABZ77676
  • Promoter PltetO-1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Superfolder GFP
  • Alt name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter PphsA

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter Bba_J23114

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD184 was a gift from Jeffrey Tabor (Addgene plasmid # 90953 ; http://n2t.net/addgene:90953 ; RRID:Addgene_90953)
  • For your References section:

    Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Daeffler KN, Galley JD, Sheth RU, Ortiz-Velez LC, Bibb CO, Shroyer NF, Britton RA, Tabor JJ. Mol Syst Biol. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416. PubMed 28373240