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(Plasmid #91751)


Item Catalog # Description Quantity Price (USD)
Plasmid 91751 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4852
  • Total vector size (bp) 5219
  • Modifications to backbone
    Nucleotides coding for Asn209 (EU numbering) were changed into triplet encoding Pro.
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Top10 or DH5alpha could be selected on LB LOW SALT with 35-50 micrograms/ml Zeocin.
  • Copy number
    High Copy


  • Gene/Insert name
    immunoglobulin heavy constant mu
  • Alt name
    IgM, IGHM, mouse IgM (immunoglobulin M) heavy chain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Asn209 changed to Pro (EU numbering)
  • Entrez Gene
    Ighm (a.k.a. Igh-6, Igh-M, Igh6, Igm, muH)
  • Promoter hEF1-HTLV prom

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUSEss-CHIg-mM-Asn209Pro_M18 was a gift from Joanna Bereta (Addgene plasmid # 91751 ; ; RRID:Addgene_91751)
  • For your References section:

    Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Klaus T, Stalinska K, Czaplicki D, Mak P, Skupien-Rabian B, Kedracka-Krok S, Wiatrowska K, Bzowska M, Machula M, Bereta J. Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1038/s41598-017-19003-4. 10.1038/s41598-017-19003-4 PubMed 29323348