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Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains.
Klaus T, Stalinska K, Czaplicki D, Mak P, Skupien-Rabian B, Kedracka-Krok S, Wiatrowska K, Bzowska M, Machula M, Bereta J
Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1038/s41598-017-19003-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
91729pFUSEss-CHIg-mM_M18Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.
91730pFUSEss-CHIg-mM-His204Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His204Ala (EU numbering).
91731pFUSEss-CHIg-mM-Tyr205Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Tyr205Ala (EU numbering).
91732pFUSEss-CHIg-mM-Lys208Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys208Ala (EU numbering).
91733pFUSEss-CHIg-mM-Asn209Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
91734pFUSEss-CHIg-mM-Arg210Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
91735pFUSEss-CHIg-mM-Asp212Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ala (EU numbering).
91736pFUSEss-CHIg-mM-Leu213Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Leu213Ala (EU numbering).
91737pFUSEss-CHIg-mM-His214Ala_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His214Ala (EU numbering).
91738pFUSEss-chimeric-IgM-mouse/human_M18Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.
91739pFUSEss-CHIg-mM-Asp212Ser_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209
91740pFUSEss-CHIg-mM-Asn209Arg_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).
91741pFUSEss-CHIg-mM-Asn209Cys_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Cys (EU numbering).
91742pFUSEss-CHIg-mM-Asn209Gln_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gln (EU numbering).
91743pFUSEss-CHIg-mM-Asn209Glu_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Glu (EU numbering).
91744pFUSEss-CHIg-mM-Asn209Gly_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gly (EU numbering).
91745pFUSEss-CHIg-mM-Asn209His_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209His (EU numbering).
91746pFUSEss-CHIg-mM-Asn209Ile_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering).
91747pFUSEss-CHIg-mM-Asn209Leu_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Leu (EU numbering).
91748pFUSEss-CHIg-mM-Asn209Lys_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Lys (EU numbering).
91749pFUSEss-CHIg-mM-Asn209Met_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Met (EU numbering).
91750pFUSEss-CHIg-mM-Asn209Phe_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Phe (EU numbering).
91751pFUSEss-CHIg-mM-Asn209Pro_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Pro (EU numbering).
91752pFUSEss-CHIg-mM-Asn209Ser_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ser (EU numbering).
91753pFUSEss-CHIg-mM-Asn209Thr_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering).
91754pFUSEss-CHIg-mM-Asn209Trp_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Trp (EU numbering).
91755pFUSEss-CHIg-mM-Asn209Tyr_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).
91756pFUSEss-CHIg-mM-Asn209Val_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering).
91781pFUSEss-CHIg-mM-Asn209Asp_M18Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

Antibodies from Article