|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91828||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
Insert Size (bp)2233
MutationNucleotide 489 of the myo-2 promoter was changed from G to C to eliminate a SapI site
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
The Pmyo-2::GFP cassette is inserted in place of a fluorescent protein in the assembled repair template. This is intended for knocking out a gene and replacing it with Pmyo-2::GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDD401 was a gift from Bob Goldstein (Addgene plasmid # 91828 ; http://n2t.net/addgene:91828 ; RRID:Addgene_91828)