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SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette.
Dickinson D, Slabodnick M, Chen A, Goldstein B
MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
91821pDD399AID CT-tag linker donor for the SapTrap cloning system
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91822pDD397AID FP-slot donor for the SapTrap cloning system
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91823pDD377Codon-optimized mTurquoise2 donor for the SapTrap cloning system
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91824pDD372Codon-optimized GFP donor for the SapTrap cloning system
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91825pDD375Codon-optimized mKate2 donor for the SapTrap cloning system
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91826pMS050Codon-optimized mScarlet-I donor for the SapTrap cloning system
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91827pDD378Codon-optimized HaloTag donor for the SapTrap cloning system
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91828pDD401Pmyo-2::GFP FP-slot donor for the SapTrap cloning system
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91829pDD363LoxP-flanked SEC donor for the SapTrap cloning system
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91830pDD382Lox2272-flanked SEC donor for the SapTrap cloning system
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91831pDD364Lox511I-flanked SEC donor for the SapTrap cloning system
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91832pDD398AID NT-tag linker donor for the SapTrap cloning system
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91833pDD121Cas9 expression in C. elegans
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91834pDD379SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold
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Antibodies from Article