SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette.
Dickinson D, Slabodnick M, Chen A, Goldstein B
MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N. PubMed Article
Dickinson D, Slabodnick M, Chen A, Goldstein B
MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 91821 | pDD399 | AID CT-tag linker donor for the SapTrap cloning system |
| 91822 | pDD397 | AID FP-slot donor for the SapTrap cloning system |
| 91823 | pDD377 | Codon-optimized mTurquoise2 donor for the SapTrap cloning system |
| 91824 | pDD372 | Codon-optimized GFP donor for the SapTrap cloning system |
| 91825 | pDD375 | Codon-optimized mKate2 donor for the SapTrap cloning system |
| 91826 | pMS050 | Codon-optimized mScarlet-I donor for the SapTrap cloning system |
| 91827 | pDD378 | Codon-optimized HaloTag donor for the SapTrap cloning system |
| 91828 | pDD401 | Pmyo-2::GFP FP-slot donor for the SapTrap cloning system |
| 91829 | pDD363 | LoxP-flanked SEC donor for the SapTrap cloning system |
| 91830 | pDD382 | Lox2272-flanked SEC donor for the SapTrap cloning system |
| 91831 | pDD364 | Lox511I-flanked SEC donor for the SapTrap cloning system |
| 91832 | pDD398 | AID NT-tag linker donor for the SapTrap cloning system |
| 91833 | pDD121 | Cas9 expression in C. elegans |
| 91834 | pDD379 | SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold |