|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91833||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerErik Jorgensen lab (Addgene #24874)
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4323
MutationCodon optimized and with synthetic introns for C. elegans
- Cloning method Gibson Cloning
- 5′ sequencing primer TCAGTTGGGAAACACTTTGCT
- 3′ sequencing primer gcttgaaaggattttgcatttatc (Common Sequencing Primers)
Same Cas9 expression cassette present in the widely-used pDD162, but does not have an sgRNA expression cassette.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDD121 was a gift from Bob Goldstein (Addgene plasmid # 91833 ; http://n2t.net/addgene:91833 ; RRID:Addgene_91833)