pDD397
(Plasmid #91822)

Purpose

AID FP-slot donor for the SapTrap cloning system
Depositing Lab

Bob Goldstein
Publication

Unpublished SapTrap/SEC plasmids (unpublished) ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	91822	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pMLS280
(Search Vector Database)
Backbone manufacturer
Erik Jorgensen lab (Addgene #73738)
Vector type
Worm Expression, CRISPR

Growth in Bacteria

Bacterial Resistance(s)
Kanamycin, 50 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
Auxin-inducible degron (AID)
Species
A. thaliana (mustard weed)
Insert Size (bp)
138

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site Unknown (unknown if destroyed)
3′ cloning site Unknown (unknown if destroyed)
5′ sequencing primer T7
3′ sequencing primer T3

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- AID FP slot donor pDD397.gb

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

The auxin-inducible degron is insertered in place of a fluorescent protein in the assembled repair template. This allows tagging a gene with AID but without an FP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pDD397 was a gift from Bob Goldstein (Addgene plasmid # 91822 ; http://n2t.net/addgene:91822 ; RRID:Addgene_91822)

pDD397 (Plasmid #91822)