PurposeAID FP-slot donor for the SapTrap cloning system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91822||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerErik Jorgensen lab (Addgene #73738)
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameAuxin-inducible degron (AID)
SpeciesA. thaliana (mustard weed)
Insert Size (bp)138
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The auxin-inducible degron is insertered in place of a fluorescent protein in the assembled repair template. This allows tagging a gene with AID but without an FP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDD397 was a gift from Bob Goldstein (Addgene plasmid # 91822 ; http://n2t.net/addgene:91822 ; RRID:Addgene_91822)
For your References section:SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette. Dickinson D, Slabodnick M, Chen A, Goldstein B. MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N. 10.17912/W2KT0N PubMed 32550377