pDD397
(Plasmid #91822)

• Purpose: AID FP-slot donor for the SapTrap cloning system
• Depositing Lab: Bob Goldstein
• Publication: Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N.

Backbone

• Vector backbone: pMLS280
  (Search Vector Database)
• Backbone manufacturer: Erik Jorgensen lab (Addgene #73738)
• Vector type: Worm Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Auxin-inducible degron (AID)
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 138

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • AID FP slot donor pDD397.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The auxin-inducible degron is insertered in place of a fluorescent protein in the assembled repair template. This allows tagging a gene with AID but without an FP