PurposeExpresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92032||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4994
- Total vector size (bp) 6830
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)1836
MutationNLS on CRY2 is mutated
Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter CMV
/ Fusion Protein
- tTA2 (TetR fused to VP64) (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer custom
- 3′ sequencing primer custom (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byModified from plasmid in Tucker lab (already available on Addgene, CRY2(deltaNLS)-mCh) which contains mCherry fluorescent protein in vector mCherryN1 (Clontech). tTA2 domain was amplified from Addgene 36430.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CRY-tTA (B702) was a gift from Chandra Tucker (Addgene plasmid # 92032 ; http://n2t.net/addgene:92032 ; RRID:Addgene_92032)
For your References section:Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260. 10.1093/nar/gkx260 PubMed 28431041