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pSFVCs-lacZ
(Plasmid #92076)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92076 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 92076-D50 50 μg of DNA in Tris buffer $495
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DNA 92076-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGEM1
  • Vector type
    C-lacZ expression plasmid, for run-off transcription and RNA transfection of mammalian cells

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C-lacZ

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Semliki forest virus (SFV) vector expression control with capsid expression enhancement. In this construct the lacZ is expressed as a C-lacZ fusion protein.

The plasmid contains the same silent mutations as pSP6-SFV4s in order to remove bacterial transcription promotors.

The capsid protein (C) of SFV contains a protease activity that cleaves the C protein off the growing polypeptide chain during biosynthesis. The C protease cleaves after the C-terminal W in the wild type sequence WSA. In recombinant proteins the C protease cleave many sequences equally well e.g. WSA, WGE, WMM, WSL and WAS. producing recombinant proteins with correct N-terminus. However, the sequence WDP, the consequence of using a BamH1 cloning site, is not cleaved.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSFVCs-lacZ was a gift from Henrik Garoff (Addgene plasmid # 92076 ; http://n2t.net/addgene:92076 ; RRID:Addgene_92076)

  • For your References section:

    A significantly improved Semliki Forest virus expression system based on translation enhancer segments from the viral capsid gene. Sjoberg EM, Suomalainen M, Garoff H. Biotechnology (N Y). 1994 Nov;12(11):1127-31. PubMed 7765556
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