PurposePI(3,4,5)P3 single molecule fluorescent reporter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92168||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Insert Size (bp)2000
- Promoter CMV
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:iRFP-PAmCherry1-PH_GRP1 was a gift from Xuelin Lou (Addgene plasmid # 92168 ; http://n2t.net/addgene:92168 ; RRID:Addgene_92168)