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pMCh-633
(Plasmid #92253)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92253 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEBG
  • Backbone manufacturer
    Addgene 22227
  • Backbone size w/o insert (bp) 5996
  • Total vector size (bp) 6965
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNRC6C CED
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    969
  • Mutation
    C-terminal effector domain (CED): aa 1369-1690 with the following point mutations: W1445A; W1487A;W1494A; W1504A; W1605A; W1648A; W1659A
  • GenBank ID
    NM_001142640.1
  • Entrez Gene
    TNRC6C
  • Promoter EF1A
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer atagcatggcctttgcaggg
  • 3′ sequencing primer ACAGGGATTTCTTGTCTCCCAC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh-633 was a gift from Marina Chekulaeva & Witold Filipowicz (Addgene plasmid # 92253 ; http://n2t.net/addgene:92253 ; RRID:Addgene_92253)
  • For your References section:

    miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W. Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166. 10.1038/nsmb.2166 PubMed 21984184