pMCh-658
(Plasmid #92257)

• Purpose: Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif
• Depositing Labs: Marina Chekulaeva, Witold Filipowicz
• Publication: Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166.

Backbone

• Vector backbone: pCiNeo-NHA
• Backbone size w/o insert (bp): 5622
• Total vector size (bp): 6591
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TNRC6C CED
• Species: H. sapiens (human)
• Insert Size (bp): 969
• Mutation: C-terminal effector domain (CED): aa 1369-1690 with the following point mutations: EF1388AA.
• GenBank ID: NM_001142640.1
• Entrez Gene: TNRC6C
• Promoter: CMV
• Tag / Fusion Protein:
  • NHA (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CACAGGTGTCCACTCCCAGTTC
• 3′ sequencing primer: ACTGCATTCTAGTTGTGGTTTGTCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry