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(Plasmid #92260)


Item Catalog # Description Quantity Price (USD)
Plasmid 92260 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5361
  • Total vector size (bp) 9525
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
  • Promoter Ac5
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACA CAA AGC CGC TCC ATC AG
  • 3′ sequencing primer CCT TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Note: Addgene's quality control sequencing finds two additional amino acid residue substitutions (V704A and N799S). The effects of these additional residue changes on protein function are not known.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh-56 was a gift from Marina Chekulaeva & Roy Parker (Addgene plasmid # 92260 ; ; RRID:Addgene_92260)
  • For your References section:

    Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila. Chekulaeva M, Filipowicz W, Parker R. RNA. 2009 May;15(5):794-803. doi: 10.1261/rna.1364909. Epub 2009 Mar 20. 10.1261/rna.1364909 PubMed 19304924