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pCMV-Tag/SP S245X with 5'UTR
(Plasmid #92370)


Item Catalog # Description Quantity Price (USD)
Plasmid 92370 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
    NM 014946
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoICR I/HincII (destroyed during cloning)
  • 3′ cloning site BamH I/Bgl II (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag/SP S245X with 5'UTR was a gift from Peter Baas (Addgene plasmid # 92370 ; ; RRID:Addgene_92370)
  • For your References section:

    Studies on truncating mutations of SPAST associated with Hereditary Spastic Paraplegia indicate greater accumulation and toxicity of the M1 isoform of spastin. Solowska JM, Rao AN, Baas PW. Mol Biol Cell. 2017 May 11. pii: mbc.E17-01-0047. doi: 10.1091/mbc.E17-01-0047. 10.1091/mbc.E17-01-0047 PubMed 28495799