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pTRE/SP M87 N184X
(Plasmid #92373)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92373 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRE-Tight
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spastin
  • Alt name
    SPAST
  • Alt name
    NM 014946
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1668
  • Mutation
    1-460 bp deleted, N184X
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter Tet-responsive P tight

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin. To detect spastin use an anti-spastin antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE/SP M87 N184X was a gift from Peter Baas (Addgene plasmid # 92373 ; http://n2t.net/addgene:92373 ; RRID:Addgene_92373)
  • For your References section:

    Studies on truncating mutations of SPAST associated with Hereditary Spastic Paraplegia indicate greater accumulation and toxicity of the M1 isoform of spastin. Solowska JM, Rao AN, Baas PW. Mol Biol Cell. 2017 May 11. pii: mbc.E17-01-0047. doi: 10.1091/mbc.E17-01-0047. 10.1091/mbc.E17-01-0047 PubMed 28495799