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FKBP-Stx4-mCitrine
(Plasmid #92427)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92427 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCitrine-N1
  • Backbone manufacturer
    Van den Bogaart lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stx4
  • Alt name
    syntaxin 4, syntaxin-4
  • Species
    R. norvegicus (rat)
  • GenBank ID
    NM_031125.1
  • Entrez Gene
    Stx4 (a.k.a. Stx4a)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCitrine (C terminal on insert)
    • FKBP (FK506 binding protein 12); allows for heterodimerization with FRB by rapamycin (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAACAACTCCGCCCCATT
  • 3′ sequencing primer GTCCAGCTCGACCAGGATGGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FKBP-Stx4-mCitrine was a gift from Geert van den Bogaart (Addgene plasmid # 92427 ; http://n2t.net/addgene:92427 ; RRID:Addgene_92427)
  • For your References section:

    Fluorescence lifetime imaging microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Verboogen DRJ, Gonzalez Mancha N, Ter Beest M, van den Bogaart G. Elife. 2017 May 19;6. pii: e23525. doi: 10.7554/eLife.23525. 10.7554/eLife.23525 PubMed 28524818