Fluorescence lifetime imaging microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Verboogen DRJ, Gonzalez Mancha N, Ter Beest M, van den Bogaart G
Elife. 2017 May 19;6. pii: e23525. doi: 10.7554/eLife.23525. PubMed Article
Verboogen DRJ, Gonzalez Mancha N, Ter Beest M, van den Bogaart G
Elife. 2017 May 19;6. pii: e23525. doi: 10.7554/eLife.23525. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92420 | pmCitrine_N1 | Empty backbone for C-terminal fusion of mCitrine |
| 92421 | Stx3-pmCitrine | Expression of syntaxin-3 C-terminally conjugated to mCitrine. |
| 92422 | Stx4-pmCitrine | Expression of syntaxin-4 C-terminally conjugated to mCitrine. |
| 92423 | VAMP3-mCherry | Expression of VAMP3 C-terminally conjugated to mCherry. |
| 92424 | VAMP8-mCherry | Expression of VAMP8 C-terminally conjugated to mCherry. |
| 92425 | VAMP3(Δ71)-mCherry | Expression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry. |
| 92426 | Stx3-mCitrine-mCherry | Expression of syntaxin-3 C-terminally conjugated to tandem of mCitrine and mCherry. |
| 92427 | FKBP-Stx4-mCitrine | Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine. |
| 92428 | FKBP-Stx3-mCitrine | Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine. |
| 92429 | FRB_T2098L-VAMP3-mCherry | Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine. |