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Fluorescence lifetime imaging microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Verboogen DRJ, Gonzalez Mancha N, Ter Beest M, van den Bogaart G
Elife. 2017 May 19;6. pii: e23525. doi: 10.7554/eLife.23525.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92420pmCitrine_N1Empty backbone for C-terminal fusion of mCitrine
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92421Stx3-pmCitrineExpression of syntaxin-3 C-terminally conjugated to mCitrine.
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92422Stx4-pmCitrineExpression of syntaxin-4 C-terminally conjugated to mCitrine.
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92423VAMP3-mCherryExpression of VAMP3 C-terminally conjugated to mCherry.
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92424VAMP8-mCherryExpression of VAMP8 C-terminally conjugated to mCherry.
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92425VAMP3(Δ71)-mCherryExpression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry.
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92426Stx3-mCitrine-mCherryExpression of syntaxin-3 C-terminally conjugated to tandem of mCitrine and mCherry.
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92427FKBP-Stx4-mCitrineExpression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.
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92428FKBP-Stx3-mCitrineExpression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.
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92429FRB_T2098L-VAMP3-mCherryExpression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.
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