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VAMP8-mCherry
(Plasmid #92424)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92424 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    CloneTech
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP8
  • Alt name
    VAMP8,endobrevin
  • Species
    M. musculus (mouse)
  • Mutation
    A36S (Please see Depositor Comments below)
  • GenBank ID
    NM_016794.3
  • Entrez Gene
    Vamp8 (a.k.a. AU041171, Edb, endobrevin)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAACAACTCCGCCCCATT
  • 3′ sequencing primer CACCTTGAAGCGCATGAACT
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the VAMP8 sequence is from Uniprot (accession number O70404). Relative to the Genbank sequence (NM_016794.3), this construct carries mutation A36S.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VAMP8-mCherry was a gift from Geert van den Bogaart (Addgene plasmid # 92424 ; http://n2t.net/addgene:92424 ; RRID:Addgene_92424)
  • For your References section:

    Fluorescence lifetime imaging microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Verboogen DRJ, Gonzalez Mancha N, Ter Beest M, van den Bogaart G. Elife. 2017 May 19;6. pii: e23525. doi: 10.7554/eLife.23525. 10.7554/eLife.23525 PubMed 28524818