pET-Duet-ECHA(37-763)-FLAG (no His-tag)
(Plasmid #96887)

• Purpose: expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)
• Depositing Lab: Hening Lin
• Publication: Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5.

Backbone

• Vector backbone: pETduet
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5420
• Total vector size (bp): 7634
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Trifunctional enzyme subunit alpha, mitochondrial
• Alt name: ECHA
• Alt name: Hadha
• Species: M. musculus (mouse)
• Insert Size (bp): 2214
• Mutation: deleted amino acid 1-36
• Entrez Gene: Hadha (a.k.a. C77020, Mtpa)
• Promoter: T7
• Tag / Fusion Protein:
  • FLAG-tagged (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: forward gene primer
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry