pet-ECHB(35-475)
(Plasmid #96890)

• Purpose: expresses His-tagged mouse ECHB (35-475) in E.coli cells
• Depositing Lab: Hening Lin
• Publication: Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5.

Backbone

• Vector backbone: pET--28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6695
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Trifunctional enzyme subunit beta, mitochondrial
• Alt name: Hadhb
• Alt name: 3-ketoacyl-CoA thiolase
• Species: M. musculus (mouse)
• Insert Size (bp): 1326
• Mutation: deleted amino acid 1-34
• Entrez Gene: Hadhb (a.k.a. 4930479F15Rik, Mtpb, TP-beta)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcorI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry