dsAAV-F4/80-MVMi-hGFP
(Plasmid #96933)

• Purpose: scAAV vector expressing humanized GFP driven by the microglia-specific promoter F4/80p. Contains minute virus of mouse intron (MVMi)
• Depositing Lab: Todd Golde
• Publication: Rosario et al Mol Ther Methods Clin Dev. 2016 Apr 13;3:16026. doi: 10.1038/mtm.2016.26. eCollection 2016.

Backbone

• Vector backbone: double-stranded sc pAAV vector
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Humanized EGFP
• Species: Synthetic
• Mutation: Humanized
• Promoter: F4/80

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A pAAV vector containing a hybrid cytomegalovirus enhancer/CBA promoter, a CBA intron (first intron of CBA gene plus the splice acceptor of the rabbit _beta-globin gene), woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the EGFP transgene was used as a template for further subcloning. This vector was modified by swapping in a modified SV40 late 16S rRNA intron in place of the CBA intron and also deleting the terminal resolution site sequence from the inverted terminal repeats to produce a mutant inverted terminal repeats, such that the viral Rep protein cannot generate the ss DNA nick. This resulted in a double-stranded sc pAAV vector. To facilitate subcloning in this sc vector, a multiple cloning site was introduced, and the SV40 intron was replaced by the minute virus of mouse intron (MVMi).