pmSTARRseq1
(Plasmid #96945)

• Purpose: (Empty Backbone) CpG free plasmid for testing effects of methylation on regulatory activity
• Depositing Lab: Jenny Tung
• Publication: Tung lab mSTARR-seq plasmud (unpublished)

Backbone

• Vector backbone: pCpGfree-promoter-Lucia_v2
• Backbone manufacturer: Invivogen
• Backbone size (bp): 2000
• Modifications to backbone: We replaced the region between the NsiI and NheI digest sites with a sequence containing the following elements: a CpG -free EF1 promoter (Invivogen), a CpG free version of a synthetic intron (pIRESpuro3, Clontech), an ORF (the CpG-free CFP::Sh gene from Invivogen), and a cluster of MluI digest sites for screening. The MluI cluster is flanked by restriction enzyme digest sites for SpeI and NcoI to enable high-throughput replacement of this sequence with putative enhancer elements.
• Vector type: Mammalian Expression ; mSTARR-seq screening vector
• Promoter: CpG -free EF1 promoter

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • InvivoGen CpGfree Limited Use Label License
• Industry Terms:
  • Not Available to Industry