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pEAHISMRSA0482
(Plasmid #96968)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 96968 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEAHISTEV, modified from pDEST14
  • Backbone manufacturer
    BSRC, University of St Andrews
  • Backbone size w/o insert (bp) 3705
  • Total vector size (bp) 5043
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein expression in DE3 strains
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MRSA ORF0482
  • Alt name
    Orn/Lys/Arg decarboxylase family protein
  • Species
    methicillin-resistant Staphylococcus aureus
  • Insert Size (bp)
    1338
  • Mutation
    none
  • Promoter T7
  • Tag / Fusion Protein
    • N-ter TEV protease cleavable 6His (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEAHISMRSA0482 was a gift from Huanting Liu & James Naismith (Addgene plasmid # 96968 ; http://n2t.net/addgene:96968 ; RRID:Addgene_96968)
  • For your References section:

    The Scottish Structural Proteomics Facility: targets, methods and outputs. Oke M, Carter LG, Johnson KA, Liu H, McMahon SA, Yan X, Kerou M, Weikart ND, Kadi N, Sheikh MA, Schmelz S, Dorward M, Zawadzki M, Cozens C, Falconer H, Powers H, Overton IM, van Niekerk CA, Peng X, Patel P, Garrett RA, Prangishvili D, Botting CH, Coote PJ, Dryden DT, Barton GJ, Schwarz-Linek U, Challis GL, Taylor GL, White MF, Naismith JH. J Struct Funct Genomics. 2010 Jun;11(2):167-80. doi: 10.1007/s10969-010-9090-y. Epub 2010 Apr 24. 10.1007/s10969-010-9090-y PubMed 20419351