p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA
(Plasmid #97010)

• Purpose: (Empty Backbone) Rosa26 targeting vector for FLP-responsive cassette
• Depositing Lab: Russell Ray
• Publication: Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0.

Backbone

• Vector backbone: p15a ori vector
• Backbone size (bp): 9204
• Vector type: Mammalian Expression, Cre/Lox, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Resource Information

• Supplemental Documents:
  • p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is for ES cell targeting of a FLP responsive cassette into the Rosa26 locus. It consists of a 5' homology arm, CAG promoter, FRT-flanked neomycin resistance and stop cassette, SwaI cloning site, WPRE and bgh pA elements, and a 3' homology arm. The cDNA of interest (expressed in response to FLP recombination) can be cloned into the SwaI site. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid.