A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping.
Lusk SJ, McKinney A, Hunt PJ, Fahey PG, Patel J, Chang A, Sun JJ, Martinez VK, Zhu PJ, Egbert JR, Allen G, Jiang X, Arenkiel BR, Tolias AS, Costa-Mattioli M, Ray RS
BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0. PubMed Article
Lusk SJ, McKinney A, Hunt PJ, Fahey PG, Patel J, Chang A, Sun JJ, Martinez VK, Zhu PJ, Egbert JR, Allen G, Jiang X, Arenkiel BR, Tolias AS, Costa-Mattioli M, Ray RS
BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 97007 | px330_Rosa_sgRNA | Expresses Cas9 and Rosa26 locus specific sgRNA |
| 97008 | p15a_Kan_MLS_Rosa26_Short_CRISPRDel | Rosa26 locus targeting vector |
| 97010 | p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA | Rosa26 targeting vector for FLP-responsive cassette |
| 97011 | p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_RNeoStop_WPRE_bgh_pA | Rosa26 targeting vector for Dre-responsive cassette |
| 97012 | p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA | Rosa26 targeting vector for intersectional Cre and FLP responsive cassette |