pcDNA5/FRT/TO-DDX54-FLAG/HA
(Plasmid #97062)

• Purpose: Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells
• Depositing Lab: Markus Landthaler
• Publication: Milek et al Genome Res. 2017 Aug;27(8):1344-1359. doi: 10.1101/gr.218438.116. Epub 2017 Jun 8.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Thermo Fischer
• Backbone size w/o insert (bp): 5137
• Total vector size (bp): 7832
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDX54
• Alt name: DP97
• Species: H. sapiens (human)
• Insert Size (bp): 2646
• GenBank ID: NM_024072.3
• Entrez Gene: DDX54 (a.k.a. DP97)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG/HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-forward
• 3′ sequencing primer: pcDNA3.1-reverse

Resource Information

• Supplemental Documents:
  • pcDNA5:FRT:TO-DDX54-FLAGHA.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry