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pFRT/TO/RFP-DDX54
(Plasmid #97061)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97061 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 97061-D50 50 μg of DNA in Tris buffer $495
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DNA 97061-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFRT/TO/RFP-DEST
  • Backbone size w/o insert (bp) 8392
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX54
  • Alt name
    DP97
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2646
  • GenBank ID
    NM_024072.3
  • Entrez Gene
    DDX54 (a.k.a. DP97)
  • Promoter CMV
  • Tag / Fusion Protein
    • RFP (tdTomato) (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer pcDNA3.1-reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/TO/RFP-DDX54 was a gift from Markus Landthaler (Addgene plasmid # 97061 ; http://n2t.net/addgene:97061 ; RRID:Addgene_97061)

  • For your References section:

    DDX54 regulates transcriptome dynamics during DNA damage response. Milek M, Imami K, Mukherjee N, Bortoli F, Zinnall U, Hazapis O, Trahan C, Oeffinger M, Heyd F, Ohler U, Selbach M, Landthaler M. Genome Res. 2017 Aug;27(8):1344-1359. doi: 10.1101/gr.218438.116. Epub 2017 Jun 8. 10.1101/gr.218438.116 PubMed 28596291
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