pFUSE-B_P2rP4
(Plasmid
#97185)
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Purpose(Empty Backbone) For fusion gene cloning. Recombine Gene B into this Gateway Entry Vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR223_P2rP4
- Backbone size (bp) 5008
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Vector typeGateway
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Modified version of pDONR223_P12 from Invitrogen.
Recombine Gateway compatibile PCR product (attB2r - attB4) into this Entry vector by Gateway BP reaction.
The pFUSE-A_P1P2 is the same plasmid as Gateway™ pDONR™221 Vector and is commercially available from ThermoFisher Scientific (Cat#: 12536017).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFUSE-B_P2rP4 was a gift from Kenneth Scott (Addgene plasmid # 97185 ; http://n2t.net/addgene:97185 ; RRID:Addgene_97185) -
For your References section:
Engineering and Functional Characterization of Fusion Genes Identifies Novel Oncogenic Drivers of Cancer. Lu H, Villafane N, Dogruluk T, Grzeskowiak CL, Kong K, Tsang YH, Zagorodna O, Pantazi A, Yang L, Neill NJ, Kim YW, Creighton CJ, Verhaak RG, Mills GB, Park P, Kucherlapati RS, Scott KL. Cancer Res. 2017 May 16. pii: canres.2745.2016. doi: 10.1158/0008-5472.CAN-16-2745. 10.1158/0008-5472.CAN-16-2745 PubMed 28512244
